Dipl.-Phys. Prof. Dr. Wolfgang Kleinekofort | Angewandte Physik & Medizintechnik

Forschungsinteressen

Extrakorporale Organersatzverfahren

Automatisierte Systeme zur kontinuierlichen Organperfusion

Sensorbasierte Feedback-Algorithmen in der Medizintechnik

Nichtkovalent gebundene biomolekulare Komplexe und Biomarker

Veröffentlichungen und Konferenzbeiträge 

Lupu, L.-M.;Wiegand, P.; Holdschick, D.; Mihoc, D.; Maeser, S.; Rawer, S.; Völklein, F.; Malek, E.; Barka, F.; Knauer, S.; Uth C.; Hennermann J.; Kleinekofort W.; Hahn A.; Barka G.; Przybylski M.: Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination. Int. J. Mol. Sci. 2021, 22, 12832; DOI: https://doi.org/10.3390/ijms222312832

Mihoc D, Lupu L, Wiegand P, Kleinekofort W, Müller O, Völklein F, Glocker M.O., Barka F, Barka G, Przybylski M: Antibody Epitope and Affinity Determination of the Myocardial Infarction Marker Myoglobin by SPR-Biosensor Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2021, 32, 1, 106–113; DOI: https://doi.org/10.1021/jasms.0c00234

Przybylski M., Kleinekofort W., Wiegand P., Lupu L.: Den Antikörpern auf der Spur. Transfer - das Steinbeis-Magazin; Ausgabe 1/2020; ISSN 1864-1768. https://transfermagazin.steinbeis.de/?p=7703

Lupu L., Wiegand P., Hüttmann N., Rawer S., Kleinekofort W., Shugorevai I., Kichkailo A.S., Tomilin F.N., Lazarev A., Berezovski M.V., Pzrybylski M.: Molecular Epitope Determination of Aptamer Complexes of the Multi‐domain Protein C‐Met by Proteolytic Affinity‐ Mass Spectrometry. ChemMedChem 2020; 15 (4), 363-369; DOI:10.1002/cmdc.201900489

W. Kleinekofort, M. Przybylski: Safety algorithms based on pattern recognition reduce false positive alarms during hemodialysis. Int J Artif Organs 2019; 42 (8): 415

B.A. Petre, L. Ion, C. Dimitriu, S. Maeser, W. Kleinekofort, C. Bulei, M. Przybylski: Clinical Diagnostics of Lysosomal Storage Diseases in DBS using new substrates by MRM-MS.  Proceedings of the 67th ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta (06/2019) 

M. Przybylski, L. Lupu, P. Wiegand, N. Hüttmann, S. Rawer, W. Kleinekofort, I. Shchugoreva, A.S. Kichkailo, F.N. Tomilin, A. Lazarev, M.V. Berezovski: Epitope Structures Of Aptamer Complexes Of The Multi-Domain Protein C-Met Revealed By Proteolytic Affinity- Mass Spectrometry. Proceedings of the 67th ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta (06/2019) 

L. Lupu, P. Wiegand, N. Hüttmann, S. Rawer, W. Kleinekofort, A. Lazarev, M. V. Berezovski, M. Przybylski: Molecular Epitope Determination of Aptamer Complexes of the Multi-domain Protein C-Met by Proteolytic Affinity- Mass Spectrometry. Proceedings of the 52nd Annual DGMS Conference, Rostock (03/2019) 

W. Kleinekofort: Changing perspectives in extracorporeal treatments - from passive machine monitoring to active physiologic patient data. Int J Artif Organs 2016; 39(7): 323 

F. Glocker, W. Kleinekofort: Systems theory applied to isolated kidneys during extracorporeal perfusion. Int J Artif Organs 2015; 38 (7): 394

O. Lebau, W. Kleinekofort: High dialyzer inlet pressure reduces effective blood flow during hemodialysis. Int J Artif Organs 2015; 38 (7): 397

O. Lebau, W. Kleinekofort: Simplified measurement of middle molecular toxin removal during continuous therapies. ASAIO J 2013; 59 (5): 512-516

F. Glocker, W. Kleinekofort: Can you measure if a kidney during ex-vivo organ perfusion is still alive? Int J Artif Organs 2013; 36 (8): 579

F. Völklein, O. Lebau, W. Kleinekofort: Thermopile chip for the assessment of metabolic heat production of small living cell colonies. Int J Artif Organs 2013; 36 (8): 572

O. Lebau, W. Kleinekofort: Dialysis efficiency with regard to middle molecular toxin reduction. Int J Artif Organs 2012; 35 (8); 577

Pike, A.C.W.,  Bullock, A.N.,  Kleinekofort, W.,  Zimmermann, T.,  Burgess-Brown, N.,  Sharpe, T.D.,  Thangaratnarajah, C.,  Keates, T.,  Ugochukwu, E.,  Bunkoczi, G.,  Uppenberg, J.,  Von Delft, F.,  Arrowsmith, C.H.,  Weigelt, J.,  Edwards, A.,  Bountra, C.,  La Thangue, N.B.,  Knapp, S.: Crystal structure of full length mouse strap (TTC5). RCSB protein data bank;  DOI: 10.2210/pdb4abn/pdb (01/2012)

Lebau O, Auzou N, Kleinekofort W: Dialyzer inlet pressure is a late indicator for filter clotting during hemodialysis. Int J Artif Organs 2011; 34 (8): 625-626

Pike, A.C.W.,   Sharpe, T.D.,   Burgess-Brown, N.,   Kleinekofort, W.,   Roos, A.K.,   Muniz, J.R.C.,   Chaikuad, A.,   Von Delft, F.,   Arrowsmith, C.H.,   Weigelt, J.,   Edwards, A.M.,   Bountra, C.,   Bullock, A.,   Knapp, S.: Crystal structure of human TTC5 (STRAP) C-terminal OB domain. https://www.thesgc.org/structures/2xvs  (10/2010)

R.Rodriguez, W.T.Kranert, , M.Ricciardi, W.Kleinekofort, F.Grünwald: Comparison of depth-contrast of a pinhole-collimator against a parallel hole-collimator for Tc-99m scintigraphy. European Journal of Nuclear Medicine & Molecular Imaging; Volume 37;Supplement 2; October 2010; S333

W. Kleinekofort, C. Mohr: Flow, pressure or recirculation: Which test is best for dual lumen catheters? Int J Artif Organs 2010; 33 (7): 424

C. Mohr, W. Kleinekofort: In-vitro Validation of Catheter Recirculation Measured by Thermal Dilution. IFMBE Proceedings 2009; Volume 25/7, 513-515

Kindgen-Milles D; Kram R; Kleinekofort W; Morgera S: Treatment of Severe Hypercalcemia Using Continuous Renal Replacement Therapy With Regional Citrate Anticoagulation; ASAIO Journal 4 (2008), Volume 54, 442-444

Kindgen-Milles D, Amman J, Kleinekofort W, Morgera S: Treatment of metabolic alkalosis during continuous renal replacement therapy with regional citrate anticoagulation; Int J Artif Organs 31; 4 (2008); 363-366

D. Kindgen-Milles, R. Kram, W. Kleinekofort: Assessment of temporary dialysis catheter performance on the basis of flow and pressure measurements in vivo and in vitro; ASAIO J 53 (2007); 351-356

W. Kleinekofort: Improved measurement of vascular access pressure; The Journal of Vascular Access 3 (2002); 58-63

W. Kleinekofort, M. Krämer, P.W. Chamney: A new safety algorithm for detection of blood loss during hemodialysis treatment; ASAIO Journal 48, 2 (2002); 174

P.W. Chamney, M. Krämer, C. Rode, W. Kleinekofort, V. Wizemann: A new technique for establishing dry weight in haemodialysis patients via whole body bioimpedance; Kidney International 61 (2002); 2250-2258

W. Kleinekofort, M. Krämer, C. Rode, V. Wizemann: Extracorporeal pressure monitoring and the detection of vascular access stenosis; International Journal of Artificial Organs 25 (2002); 45-50

W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Estimation of access flow from extracorporeal pressures; Blood Purification 19 (2001); 484

W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Estimation of access flow from extracorporeal pressures; 2^nd International Congress 2001, Vascular Access Society, London

W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Improved static pressure monitoring for the detection and classification of access stenosis; ASAIO Journal 46; 2 (2000); 206

W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Extra- to intracellular fluid ratio in normohydrated and overhydrated subjects; ASAIO Journal 46; 2 (2000); 207

W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Improved static pressure monitoring for the detection and classification of access stenosis; ASAIO Conference 2000, New York

W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Extra- to intracellular fluid ratio in normohydrated and overhydrated subjects; ASAIO Conference 2000, New York

P. Wabel, U. Moissl, S. Leonhardt, W. Kleinekofort, M. Krämer, R. Isermann: System zur Herzfrequenzauswertung während der Dialysebehandlung; Fortschritt-Bericht VDI 183 (1999), 183, 45-46

W. Kleinekofort, C. Rode, M. Krämer: Hydrostatic correction of access pressure measurements; Artificial Organs 23; 7 (1999); 652

W. Kleinekofort: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; DE 19848235 (1998)

F. Sobott, A. Wattenberg, W. Kleinekofort, A. Pfenninger, B. Brutschy: Laser desorption mass spectrometry on thin liquid jets; Fresenius Journal of Analytical Chemistry 360 (1998), 745-749 

F. Sobott, W. Kleinekofort, B. Brutschy: Cation selectivity of natural and synthetic ionophores probed with laser induced liquid beam mass spectromenty; Analytical Chemistry 69 (1997); 3587-3594 

W. Kleinekofort, M. Schweitzer, J. W. Engels, B. Brutschy: : Analysis of double-stranded oligonucleotides by laser induced liquid beam mass spectrometry ; International Journal of Mass Spectrometry and Ion Processes 163 (1997); 1L-4L 

W. Kleinekofort, A. Pfenninger, T. Plomer, C. Griesinger, B. Brutschy: Observation of noncovalent polypeptide dimers using laser induced liquid beam ionization/desorption; International Journal of Mass Spectrometry and Ion Processes 156 (1996); 195-202 

W. Kleinekofort, J. Avdiev, B. Brutschy: A new method of laser desorption mass spectrometry for the study of biological macromolecules ; International Journal of Mass Spectrometry and Ion Processes 152 (1996); 135-142 

W. Kleinekofort, L. Germeroth, J. van den Broeck, D. Schubert, H. Michel: The light- harvesting complex B800/850 from Rhodospirillum Molischianum is an octamer ; Biophysica et Biochemica Acta 1140 (1992); 102-104

Patente und Gebrauchsmuster

W. Kleinekofort: Method and device for monitoring an extracorporeal blood circuit. US-Patent 9878086B2 (01/2018)

C. König, W. Kleinekofort: Device and method for monitoring an access to a patient, in particular a vascular access in extracorporeal blood treatment. Patent 2662692, Canada (01/2015) 

C. König, W. Kleinekofort: Cannula connection monitoring. Patent 101444963, Korea (9/2014); Patent 2009527054, Japan (12/2012)

C. König, W. Kleinekofort: Device and method for monitoring an access to a patient. AU-Patent 102006042336.4 (01/2014); US-Patent 8,298,152 B2 (10/2012)

W. Kleinekofort: Reduction of false pressure alarms. Patent ZL200780026306.3; China (04/2012). Patent 5268906, Japan (05/2013)

W. Kleinekofort: Venous needle disconnect - safety device. Patent ZL200780021437.1; CN 10146642B; China (10/2012). Patent 5237269, Japan (04/2013) 

W. Kleinekofort: Blood treatment device. Patent PI0112264-9; Brasilien (05/2012)

W. Kleinekofort, A. Wüpper: Monitoring of tribological electrical voltages. Patent EP2063930B1, EU (04/2014); Patent 5286268, Japan (10/2013); Patent ZL200780033544.6,  China (05/2012).

W. Kleinekofort, A. Wüpper: Device and method for monitoring a patient access. US-Patent 8180443B1 (05/2012)

C. König, W. Kleinekofort: Connection monitoring. Patent ZL200780033121.4; China (02/2012); EAPO-Patent 015441 (08/2011)

C. König, W. Kleinekofort: Vorrichtung zur Überwachung eines Gefäßzugangs bei einer extrakorporalen Blutbehandlung. EP-Patent 2059280B1 (10/2010)

C. König, W. Kleinekofort: Device and method for monitoring an access to a patient, in particular a vascular access in extracorporeal blood treatment. US-Patent US20100241024A1 (09/2010); AU-Patent 102006042336.4 (01/2014); WO-Patent 2008/028653A2 (03/2008)

W. Kleinekofort: Method and device for monitoring an extracorporeal blood circuit; US-Patent 20090292236 A1 (11/2009)

W. Kleinekofort, J. Jonas: Device for use in an arrangement for monitoring an access to a patient, and method for monitoring a patient access, in a particular vascular access in extracorporeal blood treatment; US-Patent 20090198171 (08/2009)

W. Kleinekofort: Method and device for monitoring a blood vessel access. EP-Patent 1584339B1 (07/2009)

W. Kleinekofort: Method and device for monitoring an extracorporeal blood circuit; EP-Patent EP2040776B1 (04/2009); WO-Patent 2008/006559A1 (01/2008)

W. Kleinekofort: Device for use in an arrangement for monitoring an access to a patient, and method for monitoring a patient access, in particular a vascular access in extracorporeal blood treatment; WO 2007/140994 A1 (12/2007)

W. Kleinekofort: Verfahren zur Detektion arterieller Einlaufprobleme während einer extrakorporalen Blutbehandlung und extrakorporale Blutbehandlungsvorrichtung; DE-Patent 10033192B4 (10/2007)

W. Kleinekofort: Method and device for determining blood flow in a vascular access;  US-Patent 7172569B2 (02/2007)

W. Kleinekofort: Method and device for monitoring a blood vessel access; EP-Patent 000001584339A3 (11/2006); EP-Patent 000001584339A2 (10/2005)

W. Kleinekofort: Device for determination of blood flow in a vessel access; EP-Patent 000001044695B1 (04/2006)

W. Kleinekofort: Device for monitoring a blood vessel access; EP-Patent 000000995451B1 (12/2005)

W. Kleinekofort: Method and device for determining blood flow in a vascular access; US-Patent 020050096578A1 (05/2005); US-Patent 000006827698B1 (12/2004); EP 1044695 (2000); JP-Patent 2000325471 (2000); DE-Patent 19917197 (1999)

W. Kleinekofort: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; US-Patent 000006595942B2 (07/2003); JP-Patent 2000140101 (2000); EP-Patent 0995451 (1999); US-Patent 6221040 (1999); DE 19848235 (1998)

W. Kleinekofort: Vorrichtung zur Konnektionshilfe (Gebrauchsmuster); DE20214415U1 (04/2003)

W. Kleinekofort: Method for detecting arterial flow problems during extracorporeal blood treatment; WO-Patent 002002004044A9 (09/2002)

W. Kleinekofort: Verfahren zur Detektion arterieller Einlaufprobleme während einer extrakorporalen Blutbehandlung und extrakorporale Blutbehandlungsvorrichtung ; DE-Patent 000010033192A1 (01/2002)

W. Kleinekofort: Arteriovenöse Gefäßprothese; DE-Gebrauchsmuster 20100443 (2001)