Dipl.-Phys. Prof. Dr. Wolfgang Kleinekofort | Angewandte Physik & Medizintechnik

Forschungsinteressen

Extrakorporale Organersatzverfahren

Automatisierte Systeme zur kontinuierlichen Organperfusion

Sensorbasierte Feedback-Algorithmen in der Medizintechnik

Nichtkovalent gebundene biomolekulare Komplexe und Biomarker

Veröffentlichungen und Konferenzbeiträge 

Lupu L, Kleinekofort W, Morgner N: Epitope characterization of proteins and aptamers with mass spectrometry. Eur J Mass Spectrom 2023, 29, Vol. 5-6; https://doi.org/10.1177/14690667231208530

Choirot G, Kleinekofort W: Hydrodynamic behavior of vascular stenoses. 49th ESAO - IFAO Annual Congress 2023 (Poster)

Choirot G, Kleinekofort W: Analysis of filtration and backfiltration in hollow fiber membrane bioreactors. 49th ESAO - IFAO Annual Congress 2023 (Poster)

Janko M, Santaniello SD, Brockmann C, Wolf M, Grauhan NF, Schöffling VI, Dimova V, Ponto K, Hoffmann EM, Kleinekofort W, Othman A, Brockmann M, Kronfeld A: Spatial alignment of structural images on single-shot and readout-segmented (RESOLVE) diffusion images in the optic nerve for tractography template generation. Preprint from Authorea Preprints, 15 Mar 2023; https://europepmc.org/article/ppr/ppr631187

Lupu L, Wiegand P, Mihoc D, Hüttmann N, Völklein F, Barka F, Knauer S, Uth C, Kleinekofort W, Barca G, Maeser S, Przybylski M: Epitope and affinity determination of protein-antibody interactions by affinity-mass spectrometry. Wiley Analytical Science Magazine, Spectroscopy 04/2023; https://analyticalscience.wiley.com/do/10.1002/was.00050417

Choirot G, Kleinekofort W: Hidden convective flow in hollow fiber cell reactors. ESAO Winter School - Artificial Organs and Tissue Engineering (Poster); Wittenberg (02/2023)

Wiegand P, Hüttmann N, Völklein F, Knauer S, Uth C, Kleinekofort W, Barka G, Maeser S, Przybylski M, Lupu L, Mihoc D: Epitop- und Affinitätsbestimmung von Protein-Antikörper-Wechselwirkungen mittels Affinitäts-Massenspektrometrie. GIT Labor-Fachzeitschrift 1–2/2023; https://analyticalscience.wiley.com/do/10.1002/was.000600448/full/

Przybylski M, Hüttmann N, Lupu L, Wiegand P, Rawer S, Kleinekofort W, Berezovski M, Vogel M, Suess B: Epitope determination of DNA and RNA aptamers as antibody alternatives by affinity- mass spectrometry open new perspectives for peptide biomarkers and molecular diagnostics. Proceedings of the 36th European Peptide Symposium 2022; https://doi.org/10.17952/36EPS.2022.275

Lupu L, Wiegand P, Holdschick D, Mihoc D, Maeser S, Rawer S, Völklein F, Malek E, Barka F, Knauer S, Uth C, Hennermann J, Kleinekofort W, Hahn A, Barka G, Przybylski M: Identification and Affinity Determination of Protein-Antibody and Protein-Aptamer Epitopes by Biosensor-Mass Spectrometry Combination. Int. J. Mol. Sci. 2021, 22, 12832; https://doi.org/10.3390/ijms222312832

Mihoc D, Lupu L, Wiegand P, Kleinekofort W, Müller O, Völklein F, Glocker M.O., Barka F, Barka G, Przybylski M: Antibody Epitope and Affinity Determination of the Myocardial Infarction Marker Myoglobin by SPR-Biosensor Mass Spectrometry. J. Am. Soc. Mass Spectrom. 2021, 32, 1, 106–113; https://doi.org/10.1021/jasms.0c00234

Przybylski M, Kleinekofort W, Wiegand P, Lupu L: Den Antikörpern auf der Spur. Transfer - das Steinbeis-Magazin; Ausgabe 1/2020; ISSN 1864-1768. https://transfermagazin.steinbeis.de/?p=7703

Lupu L, Wiegand P, Hüttmann N, Rawer S, Kleinekofort W, Shugorevai I, Kichkailo AS, Tomilin FN, Lazarev A, Berezovski MV, Pzrybylski M: Molecular Epitope Determination of Aptamer Complexes of the Multi‐domain Protein C‐Met by Proteolytic Affinity‐ Mass Spectrometry. ChemMedChem 2020; 15 (4), 363-369; 10.1002/cmdc.201900489

Kleinekofort W, Przybylski M: Safety algorithms based on pattern recognition reduce false positive alarms during hemodialysis. Int J Artif Organs 2019; 42 (8): 415

Petre B, Ion L, Dimitriu C, Maeser S, Kleinekofort W, Bulei C, Przybylski M: Clinical Diagnostics of Lysosomal Storage Diseases in DBS using new substrates by MRM-MS.  Proceedings of the 67th ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta (06/2019) 

Przybylski M, Lupu L, Wiegand P, Hüttmann N, Rawer S, Kleinekofort W, Shchugoreva I, Kichkailo A, Tomilin F, Lazarev A, Berezovski M: Epitope Structures Of Aptamer Complexes Of The Multi-Domain Protein C-Met Revealed By Proteolytic Affinity- Mass Spectrometry. Proceedings of the 67th ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta (06/2019) 

Lupu L, Wiegand P, Hüttmann N, Rawer S, Kleinekofort W, Lazarev A, Berezovski M, Przybylski M: Molecular Epitope Determination of Aptamer Complexes of the Multi-domain Protein C-Met by Proteolytic Affinity- Mass Spectrometry. Proceedings of the 52nd Annual DGMS Conference, Rostock (03/2019) 

Kleinekofort W: Changing perspectives in extracorporeal treatments - from passive machine monitoring to active physiologic patient data. Int J Artif Organs 2016; 39(7): 323 

Glocker F, Kleinekofort W: Systems theory applied to isolated kidneys during extracorporeal perfusion. Int J Artif Organs 2015; 38 (7): 394

Lebau O, Kleinekofort W: High dialyzer inlet pressure reduces effective blood flow during hemodialysis. Int J Artif Organs 2015; 38 (7): 397

Lebau O, Kleinekofort W: Simplified measurement of middle molecular toxin removal during continuous therapies. ASAIO J 2013; 59 (5): 512-516

Glocker F, Kleinekofort W: Can you measure if a kidney during ex-vivo organ perfusion is still alive? Int J Artif Organs 2013; 36 (8): 579

Völklein F, Lebau O, Kleinekofort W: Thermopile chip for the assessment of metabolic heat production of small living cell colonies. Int J Artif Organs 2013; 36 (8): 572

Lebau O, Kleinekofort W: Dialysis efficiency with regard to middle molecular toxin reduction. Int J Artif Organs 2012; 35 (8); 577

Pike A,  Bullock A,  Kleinekofort W,  Zimmermann T,  Burgess-Brown N,  Sharpe T,  Thangaratnarajah C,  Keates T,  Ugochukwu E,  Bunkoczi G,  Uppenberg J,  Von Delft F,  Arrowsmith C,  Weigelt J,  Edwards A,  Bountra C,  La Thangue N,  Knapp S: Crystal structure of full length mouse strap (TTC5). RCSB protein data bank (01/2012); 10.2210/pdb4abn/pdb 

Lebau O, Auzou N, Kleinekofort W: Dialyzer inlet pressure is a late indicator for filter clotting during hemodialysis. Int J Artif Organs 2011; 34 (8): 625-626

Pike A, Sharpe T, Burgess-Brown N, Kleinekofort W, Roos A, Muniz J, Chaikuad A, Von Delft F, Arrowsmith C, Weigelt J, Edwards A, Bountra C, Bullock A, Knapp S: Crystal structure of human TTC5 (STRAP) C-terminal OB domain (10/2010). https://www.thesgc.org/structures/2xvs 

Rodriguez R, Kranert W, Ricciardi M, Kleinekofort W, Grünwald F: Comparison of depth-contrast of a pinhole-collimator against a parallel hole-collimator for Tc-99m scintigraphy. European Journal of Nuclear Medicine & Molecular Imaging; Volume 37;Supplement 2; October 2010; S333

Kleinekofort W, Mohr C: Flow, pressure or recirculation: Which test is best for dual lumen catheters? Int J Artif Organs 2010; 33 (7): 424

Mohr C, Kleinekofort W: In-vitro Validation of Catheter Recirculation Measured by Thermal Dilution. IFMBE Proceedings 2009; Volume 25/7, 513-515

Kindgen-Milles D, Kram R, Kleinekofort W, Morgera S: Treatment of Severe Hypercalcemia Using Continuous Renal Replacement Therapy With Regional Citrate Anticoagulation; ASAIO Journal 4 (2008), Volume 54, 442-444

Kindgen-Milles D, Amman J, Kleinekofort W, Morgera S: Treatment of metabolic alkalosis during continuous renal replacement therapy with regional citrate anticoagulation; Int J Artif Organs 31; 4 (2008); 363-366

Kindgen-Milles D, Kram R, Kleinekofort W: Assessment of temporary dialysis catheter performance on the basis of flow and pressure measurements in vivo and in vitro; ASAIO J 53 (2007); 351-356

Kleinekofort W: Improved measurement of vascular access pressure; The Journal of Vascular Access 3 (2002); 58-63

Kleinekofort W, Krämer M, Chamney PW: A new safety algorithm for detection of blood loss during hemodialysis treatment; ASAIO Journal 48, 2 (2002); 174

Chamney PW, Krämer M, Rode C, Kleinekofort W, Wizemann V: A new technique for establishing dry weight in haemodialysis patients via whole body bioimpedance; Kidney International 61 (2002); 2250-2258

Kleinekofort W, Krämer M, Rode C, Wizemann V: Extracorporeal pressure monitoring and the detection of vascular access stenosis; International Journal of Artificial Organs 25 (2002); 45-50

Kleinekofort W, Rode C, Krämer M, Wizemann V: Estimation of access flow from extracorporeal pressures; Blood Purification 19 (2001); 484

Kleinekofort W, Rode C, Krämer M, Wizemann V: Estimation of access flow from extracorporeal pressures; 2^nd International Congress 2001, Vascular Access Society, London

Kleinekofort W, Rode C, Krämer M, Wizemann V: Improved static pressure monitoring for the detection and classification of access stenosis; ASAIO Journal 46; 2 (2000); 206

Kleinekofort W, Rode C, Krämer M, Wizemann V: Extra- to intracellular fluid ratio in normohydrated and overhydrated subjects; ASAIO Journal 46; 2 (2000); 207

Kleinekofort W, Rode C, Krämer M, Wizemann V: Improved static pressure monitoring for the detection and classification of access stenosis; ASAIO Conference 2000, New York

Kleinekofort W, Rode C, Krämer M, Wizemann V: Extra- to intracellular fluid ratio in normohydrated and overhydrated subjects; ASAIO Conference 2000, New York

Wabel P, Moissl U, Leonhardt S, Kleinekofort W, Krämer M, Isermann R: System zur Herzfrequenzauswertung während der Dialysebehandlung; Fortschritt-Bericht VDI 183 (1999), 183, 45-46

Kleinekofort W, Rode C, Krämer M: Hydrostatic correction of access pressure measurements; Artificial Organs 23; 7 (1999); 652

Sobott F, Wattenberg A, Kleinekofort W, Pfenninger A, Brutschy B: Laser desorption mass spectrometry on thin liquid jets; Fresenius Journal of Analytical Chemistry 360 (1998), 745-749 

Sobott F, Kleinekofort W, Brutschy B: Cation selectivity of natural and synthetic ionophores probed with laser induced liquid beam mass spectromenty; Analytical Chemistry 69 (1997); 3587-3594 

Kleinekofort W, Schweitzer M, Engels JW, Brutschy B: Analysis of double-stranded oligonucleotides by laser induced liquid beam mass spectrometry; International Journal of Mass Spectrometry and Ion Processes 163 (1997); 1L-4L 

Kleinekofort W, Pfenninger A, Plomer T, Griesinger C, Brutschy B: Observation of noncovalent polypeptide dimers using laser induced liquid beam ionization/desorption; International Journal of Mass Spectrometry and Ion Processes 156 (1996); 195-202 

Kleinekofort W, Avdiev J, Brutschy B: A new method of laser desorption mass spectrometry for the study of biological macromolecules; International Journal of Mass Spectrometry and Ion Processes 152 (1996); 135-142 

Kleinekofort W, Germeroth L, van den Broeck J, Schubert D, Michel H: The light- harvesting complex B800/850 from Rhodospirillum Molischianum is an octamer; Biophysica et Biochemica Acta 1140 (1992); 102-104

Patente und Gebrauchsmuster

Kleinekofort W: Method and device for monitoring an extracorporeal blood circuit. US-Patent 9878086B2 (01/2018)

König C, Kleinekofort W: Device and method for monitoring an access to a patient, in particular a vascular access in extracorporeal blood treatment. Patent 2662692, Canada (01/2015) 

König C, Kleinekofort W: Cannula connection monitoring. Patent 101444963, Korea (9/2014); Patent 2009527054, Japan (12/2012)

König C, Kleinekofort W: Device and method for monitoring an access to a patient. AU-Patent 102006042336.4 (01/2014); US-Patent 8,298,152 B2 (10/2012)

Kleinekofort W: Reduction of false pressure alarms. Patent ZL200780026306.3; China (04/2012). Patent 5268906, Japan (05/2013)

Kleinekofort W: Venous needle disconnect - safety device. Patent ZL200780021437.1; CN 10146642B; China (10/2012). Patent 5237269, Japan (04/2013) 

Kleinekofort W: Blood treatment device. Patent PI0112264-9; Brasilien (05/2012)

Kleinekofort W, Wüpper A: Monitoring of tribological electrical voltages. Patent EP2063930B1, EU (04/2014); Patent 5286268, Japan (10/2013); Patent ZL200780033544.6,  China (05/2012).

Kleinekofort W, Wüpper A: Device and method for monitoring a patient access. US-Patent 8180443B1 (05/2012)

König C, Kleinekofort W: Connection monitoring. Patent ZL200780033121.4; China (02/2012); EAPO-Patent 015441 (08/2011)

König C, Kleinekofort W: Vorrichtung zur Überwachung eines Gefäßzugangs bei einer extrakorporalen Blutbehandlung. EP-Patent 2059280B1 (10/2010)

König C, Kleinekofort W: Device and method for monitoring an access to a patient, in particular a vascular access in extracorporeal blood treatment. US-Patent US20100241024A1 (09/2010); AU-Patent 102006042336.4 (01/2014); WO-Patent 2008/028653A2 (03/2008)

Kleinekofort W: Method and device for monitoring an extracorporeal blood circuit; US-Patent 20090292236 A1 (11/2009)

Kleinekofort W, Jonas J: Device for use in an arrangement for monitoring an access to a patient, and method for monitoring a patient access, in a particular vascular access in extracorporeal blood treatment; US-Patent 20090198171 (08/2009)

Kleinekofort W: Method and device for monitoring a blood vessel access. EP-Patent 1584339B1 (07/2009)

Kleinekofort W: Method and device for monitoring an extracorporeal blood circuit; EP-Patent EP2040776B1 (04/2009); WO-Patent 2008/006559A1 (01/2008)

Kleinekofort W: Device for use in an arrangement for monitoring an access to a patient, and method for monitoring a patient access, in particular a vascular access in extracorporeal blood treatment; WO 2007/140994 A1 (12/2007)

Kleinekofort W: Verfahren zur Detektion arterieller Einlaufprobleme während einer extrakorporalen Blutbehandlung und extrakorporale Blutbehandlungsvorrichtung; DE-Patent 10033192B4 (10/2007)

Kleinekofort W: Method and device for determining blood flow in a vascular access;  US-Patent 7172569B2 (02/2007)

Kleinekofort W: Method and device for monitoring a blood vessel access; EP-Patent 000001584339A3 (11/2006); EP-Patent 000001584339A2 (10/2005)

Kleinekofort W: Device for determination of blood flow in a vessel access; EP-Patent 000001044695B1 (04/2006)

Kleinekofort W: Device for monitoring a blood vessel access; EP-Patent 000000995451B1 (12/2005)

Kleinekofort W: Method and device for determining blood flow in a vascular access; US-Patent 020050096578A1 (05/2005); US-Patent 000006827698B1 (12/2004); EP 1044695 (2000); JP-Patent 2000325471 (2000); DE-Patent 19917197 (1999)

Kleinekofort W: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; US-Patent 000006595942B2 (07/2003); JP-Patent 2000140101 (2000); EP-Patent 0995451 (1999); US-Patent 6221040 (1999); DE 19848235 (1998)

Kleinekofort W: Vorrichtung zur Konnektionshilfe (Gebrauchsmuster); DE20214415U1 (04/2003)

Kleinekofort W: Method for detecting arterial flow problems during extracorporeal blood treatment; WO-Patent 002002004044A9 (09/2002)

Kleinekofort W: Verfahren zur Detektion arterieller Einlaufprobleme während einer extrakorporalen Blutbehandlung und extrakorporale Blutbehandlungsvorrichtung ; DE-Patent 000010033192A1 (01/2002)

Kleinekofort W: Arteriovenöse Gefäßprothese; DE-Gebrauchsmuster 20100443 (2001)

Kleinekofort W: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; DE 19848235C1 (1998)