Dipl.-Phys. Prof. Dr. Wolfgang Kleinekofort

Dipl.-Phys. Prof. Dr. Wolfgang Kleinekofort | Medizintechnik

Postanschrift :

Postfach 3251
65022 Wiesbaden

Besuchsadresse :

Am Brückweg 26
65428 Rüsselsheim

Sprechzeiten :

nach Vereinbarung

Lebenslauf

seit 2018

Stellvertretender Geschäftsführer, Steinbeis-Zentrum Biopolymeranalytik und Biomedizinische Massenspektrometrie

2012 - 2018

Dekan, Fachbereich Ingenieurwissenschaften

Seit 2003

Professor für Medizintechnik, Fachbereich Ingenieurwissenschaften. Wissenschaftlicher Berater im Gesundheitswesen

2001 - 2003

Project Manager, Research & Development, Fresenius Medical Care AG

1997 - 2001

Wissenschaftler, Research & Development, Fresenius Medical Care AG 

1996 - 1997

Postdoctoral Fellow, National Institute for Advanced Interdisciplinary Research, Tsukuba (Japan) 

1993 - 1996

Doktorand, Johann Wolfgang Goethe-Universität Frankfurt. Promotion zum Doktor der Naturwissenschaften mit Auszeichnung in der Arbeitsgruppe von Prof. Dr. B. Brutschy.

1988 - 1993

Physikstudium,  Johann Wolfgang Goethe-Universität Frankfurt

 

AUSZEICHNUNGEN

Tierschutzforschungspreis des Landes Hessen; 2012

Forschungspreis der Hessischen Hochschulen für Angewandte Wissenschaften (2. Platz); 2012

Gastwissenschaftler, Structural Genomics Consortium, University of Oxford, UK; 2007

Mattauch-Herzog-Promotionspreis, Deutsche Gesellschaft für Massenspektrometrie; 1997

Research Fellowship, Ministry of International Trade and Industry (MITI), Japan; 1996

Heraeus-Förderpreis, WE-Heraeus-Stiftung; 1994

 

Veröffentlichungen, Vorträge, Poster und Patente aus den Bereichen Medizingerätetechnik und Lebenswissenschaften

80) Lupu L., Wiegand P., Hüttmann N., Rawer S., Kleinekofort W., Shugorevai I., Kichkailo A.S., Tomilin F.N., Lazarev A., Berezovski M.V., Pzrybylski M. (2019): Molecular Epitope Determination of Aptamer Complexes of the Multi‐domain Protein C‐Met by Proteolytic Affinity‐ Mass Spectrometry. ChemMedChem.DOI:10.1002/cmdc.201900489

79) W. Kleinekofort, M. Przybylski: Safety algorithms based on pattern recognition reduce false positive alarms during hemodialysis. Int J Artif Organs 2019; 42 (8): 415

78) B.A. Petre, L. Ion, C. Dimitriu, S. Maeser, W. Kleinekofort, C. Bulei, M. Przybylski: Clinical Diagnostics of Lysosomal Storage Diseases in DBS using new substrates by MRM-MS.  Proceedings of the 67thASMS Conference on Mass Spectrometry and Allied Topics, Atlanta (06/2019) 

77) M. Przybylski, L. Lupu, P. Wiegand, N. Hüttmann, S. Rawer, W. Kleinekofort, I. Shchugoreva, A.S. Kichkailo, F.N. Tomilin, A. Lazarev, M.V. Berezovski: Epitope Structures Of Aptamer Complexes Of The Multi-Domain Protein C-Met Revealed By Proteolytic Affinity- Mass Spectrometry. Proceedings of the 67th ASMS Conference on Mass Spectrometry and Allied Topics, Atlanta (06/2019) 

76) L. Lupu, P. Wiegand, N. Hüttmann, S. Rawer, W. Kleinekofort, A. Lazarev, M. V. Berezovski, M. Przybylski: Molecular Epitope Determination of Aptamer Complexes of the Multi-domain Protein C-Met by Proteolytic Affinity- Mass Spectrometry. Proceedings of the 52nd Annual DGMS Conference, Rostock (03/2019) 

75) W. Kleinekofort: Method and device for monitoring an extracorporeal blood circuit. US-Patent 9878086B2 (01/2018)

74) W. Kleinekofort: Changing perspectives in extracorporeal treatments - from passive machine monitoring to active physiologic patient data. Int J Artif Organs 2016; 39(7): 323 

73) F. Glocker, W. Kleinekofort: Systems theory applied to isolated kidneys during extracorporeal perfusion. Int J Artif Organs 2015; 38 (7): 394

72) O. Lebau, W. Kleinekofort: High dialyzer inlet pressure reduces effective blood flow during hemodialysis. Int J Artif Organs 2015; 38 (7): 397

71) C. König, W. Kleinekofort: Device and method for monitoring an access to a patient, in particular a vascular access in extracorporeal blood treatment. Patent 2662692, Canada (01/2015) 

70) C. König, W. Kleinekofort: Cannula connection monitoring. Patent 101444963, Korea (9/2014)

69) W. Kleinekofort, A. Wüpper: Monitoring of tribological electrical voltages. Patent EP2063930B1; EU (04/2014)

68) C. König, W. Kleinekofort: Device and method for monitoring an access to a patient. AU-Patent 102006042336.4 (01/2014) 

67) O. Lebau, W. Kleinekofort: Simplified measurement of middle molecular toxin removal during continuous therapies. ASAIO J 2013; 59 (5): 512-516

66) F. Glocker, W. Kleinekofort: Can you measure if a kidney during ex-vivo organ perfusion is still alive? Int J Artif Organs 2013; 36 (8): 579

65) F. Völklein, O. Lebau, W. Kleinekofort: Thermopile chip for the assessment of metabolic heat production of small living cell colonies. Int J Artif Organs 2013; 36 (8): 572

64) W. Kleinekofort: Reduction of false pressure alarms. Patent ZL200780026306.3; China (04/2012). Patent 5268906, Japan (05/2013)

63) W. Kleinekofort: Venous needle disconnect - safety device. Patent ZL200780021437.1; CN 10146642B; China (10/2012). Patent 5237269, Japan (04/2013) 

62) C. König, W. Kleinekofort: Cannula connection monitoring. Patent 2009527054, Japan (12/2012)

61) C. König, W. Kleinekofort: Device and method for monitoring an access to a patient. US-Patent 8,298,152 B2 (10/2012)

60) O. Lebau, W. Kleinekofort: Dialysis efficiency with regard to middle molecular toxin reduction. Int J Artif Organs 2012; 35 (8); 577

59) W. Kleinekofort: Blood treatment device. Patent PI0112264-9; Brasilien (05/2012)

58) W. Kleinekofort, A. Wüpper: Monitoring of tribological electrical voltages. Patent ZL200780033544.6; China (05/2012). Patent 5286268; Japan (10/2013). Patent EP2063930B1; EU (04/2014)

57) W. Kleinekofort, A. Wüpper: Device and method for monitoring a patient access. US-Patent 8180443B1 (05/2012)

56) C. König, W. Kleinekofort: Connection monitoring. Patent ZL200780033121.4; China (02/2012)

55) Pike, A.C.W.,  Bullock, A.N.,  Kleinekofort, W.,  Zimmermann, T.,  Burgess-Brown, N.,  Sharpe, T.D.,  Thangaratnarajah, C.,  Keates, T.,  Ugochukwu, E.,  Bunkoczi, G.,  Uppenberg, J.,  Von Delft, F.,  Arrowsmith, C.H.,  Weigelt, J.,  Edwards, A.,  Bountra, C.,  La Thangue, N.B.,  Knapp, S.: Crystal structure of full length mouse strap (TTC5). RCSB protein data bank;  DOI: 10.2210/pdb4abn/pdb (01/2012)

54) C. König, W. Kleinekofort: Cannula connection monitoring. EAPO-Patent 015441 (08/2011)

53) Lebau O, Auzou N, Kleinekofort W: Dialyzer inlet pressure is a late indicator for filter clotting during hemodialysis. Int J Artif Organs 2011; 34 (8): 625-626

52) Pike, A.C.W.,   Sharpe, T.D.,   Burgess-Brown, N.,   Kleinekofort, W.,   Roos, A.K.,   Muniz, J.R.C.,   Chaikuad, A.,   Von Delft, F.,   Arrowsmith, C.H.,   Weigelt, J.,   Edwards, A.M.,   Bountra, C.,   Bullock, A.,   Knapp, S.: Crystal structure of human TTC5 (STRAP) C-terminal OB domain. https://www.thesgc.org/structures/2xvs  (10/2010)

51) C. König, W. Kleinekofort: Vorrichtung zur Überwachung eines Gefässzugangs bei einer extrakorporalen Blutbehandlung. EP-Patent 2059280B1 (10/2010)

50) R.Rodriguez, W.T.Kranert, , M.Ricciardi, W.Kleinekofort, F.Grünwald: Comparison of depth-contrast of a pinhole-collimator against a parallel hole-collimator for Tc-99m scintigraphy. European Journal of Nuclear Medicine & Molecular Imaging; Volume 37;Supplement 2; October 2010; S333

49) C. König, W. Kleinekofort: Device and method for monitoring an access to a patient, in particular a vascular access in extracorporeal blood treatment. US-Patent US20100241024A1 (09/2010); AU-Patent 102006042336.4 (01/2014) 

48) W. Kleinekofort, C. Mohr: Flow, pressure or recirculation: Which test is best for dual lumen catheters? Int J Artif Organs 2010; 33 (7): 424

47) C. Mohr, W. Kleinekofort: In-vitro Validation of Catheter Recirculation Measured by Thermal Dilution. IFMBE Proceedings 2009; Volume 25/7, 513-515

46) W. Kleinekofort: Method and device for monitoring an extracorporeal blood circuit; US-Patent 20090292236 A1 (11/2009)

45) W. Kleinekofort, J. Jonas: Device for use in an arrangement for monitoring an access to a patient, and method for monitoring a patient access, in a particular vascular access in extracorporeal blood treatment; US-Patent 20090198171 (08/2009)

44) W. Kleinekofort: Method and device for monitoring a blood vessel access. EP-Patent 1584339B1 (07/2009)

43) W. Kleinekofort: Method and device for monitoring an extracorporeal blood circuit; EP-Patent EP2040776B1 (04/2009)

42) Kindgen-Milles D; Kram R; Kleinekofort W; Morgera S: Treatment of Severe Hypercalcemia Using Continuous Renal Replacement Therapy With Regional Citrate Anticoagulation; ASAIO Journal 4 (2008), Volume 54, 442-444

41) Kindgen-Milles D, Amman J, Kleinekofort W, Morgera S: Treatment of metabolic alkalosis during continuous renal replacement therapy with regional citrate anticoagulation; Int J Artif Organs 31; 4 (2008); 363-366

40) C. König, W. Kleinekofort: Device and method for monitoring an access to a patient, in particular a vascular access in extracorporeal blood treatment. WO-Patent 2008/028653A2 (03/2008)

39) W. Kleinekofort: Method and device for monitoring an extracorporeal ciruit. WO-Patent 2008/006559A1 (01/2008)

38) W. Kleinekofort: Device for use in an arrangement for monitoring an access to a patient, and method for monitoring a patient access, in particular a vascular access in extracorporeal blood treatment; WO 2007/140994 A1 (12/2007)

37) W. Kleinekofort: Verfahren zur Detektion arterieller Einlaufprobleme während einer extrakorporalen Blutbehandlung und extrakorporale Blutbehandlungsvorrichtung; DE-Patent 10033192B4 (10/2007)

36) D. Kindgen-Milles, R. Kram, W. Kleinekofort: Assessment of temporary dialysis catheter performance on the basis of flow and pressure measurements in vivo and in vitro; ASAIO J 53 (2007); 351-356

35) W. Kleinekofort: Method and device for determining blood flow in a vascular access;  US-Patent 7172569B2 (02/2007)

34) W. Kleinekofort: Method and device for monitoring a blood vessel access; EP-Patent 000001584339A3 (11/2006)

33) W. Kleinekofort: Device for determination of blood flow in a vessel access; EP-Patent 000001044695B1 (04/2006)

32) W. Kleinekofort: Device for monitoring a blood vessel access; EP-Patent 000000995451B1 (12/2005)

31) W. Kleinekofort: Method and device for monitoring a blood vessel access; EP-Patent 000001584339A2 (10/2005)

30) W. Kleinekofort: Method and device for determining blood flow in a vascular access; US-Patent 020050096578A1 (05/2005)

29) W. Kleinekofort: Method and device for determining blood flow in a vascular access ; US-Patent 000006827698B1 (12/2004)

28) W. Kleinekofort: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; US-Patent 000006595942B2 (07/2003)

27) W. Kleinekofort: Vorrichtung zur Konnektionshilfe (Gebrauchsmuster); DE20214415U1 (04/2003)

26) W. Kleinekofort: Method for detecting arterial flow problems during extracorporeal blood treatment; WO-Patent 002002004044A9 (09/2002)

25) W. Kleinekofort: Verfahren zur Detektion arterieller Einlaufprobleme während einer extrakorporalen Blutbehandlung und extrakorporale Blutbehandlungsvorrichtung ; DE-Patent 000010033192A1 (01/2002)

24) W. Kleinekofort: Improved measurement of vascular access pressure; The Journal of Vascular Access 3 (2002); 58-63

23) W. Kleinekofort, M. Krämer, P.W. Chamney: A new safety algorithm for detection of blood loss during hemodialysis treatment; ASAIO Journal 48, 2 (2002); 174

22) P.W. Chamney, M. Krämer, C. Rode, W. Kleinekofort, V. Wizemann: A new technique for establishing dry weight in haemodialysis patients via whole body bioimpedance; Kidney International 61 (2002); 2250-2258

21) W. Kleinekofort, M. Krämer, C. Rode, V. Wizemann: Extracorporeal pressure monitoring and the detection of vascular access stenosis; International Journal of Artificial Organs 25 (2002); 45-50

20) W. Kleinekofort: Arteriovenöse Gefäßprothese; DE-Gebrauchsmuster 20100443 (2001)

19) W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Estimation of access flow from extracorporeal pressures; Blood Purification 19 (2001); 484

18) W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Estimation of access flow from extracorporeal pressures; 2nd International Congress 2001, Vascular Access Society, London

17) W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Improved static pressure monitoring for the detection and classification of access stenosis; ASAIO Journal 46; 2 (2000); 206

16) W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Extra- to intracellular fluid ratio in normohydrated and overhydrated subjects; ASAIO Journal 46; 2 (2000); 207

15) W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Improved static pressure monitoring for the detection and classification of access stenosis; ASAIO Conference 2000, New York

14) W. Kleinekofort, C. Rode, M. Krämer, V. Wizemann: Extra- to intracellular fluid ratio in normohydrated and overhydrated subjects; ASAIO Conference 2000, New York

13) W. Kleinekofort: Method and device for determination of blood flow in a vessel access; EP 1044695 (2000); JP-Patent 2000325471 (2000)

12) W. Kleinekofort: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; JP-Patent 2000140101 (2000)

11) W. Kleinekofort: Method and device for determination of blood flow in a vessel access; DE-Patent 19917197 (1999)

10) W. Kleinekofort: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; EP-Patent 0995451 (1999); US-Patent 6221040 (1999)

9) P. Wabel, U. Moissl, S. Leonhardt, W. Kleinekofort, M. Krämer, R. Isermann: System zur Herzfrequenzauswertung während der Dialysebehandlung; Fortschritt-Bericht VDI 183 (1999), 183, 45-46

8) W. Kleinekofort, C. Rode, M. Krämer: Hydrostatic correction of access pressure measurements; Artificial Organs 23; 7 (1999); 652

7) W. Kleinekofort: Method of monitoring a vascular access and an apparatus for extracorporeal treatment of blood with a device for monitoring the vascular access; DE 19848235 (1998)

 

Naturwissenschaftliche Veröffentlichungen

6) F. Sobott, A. Wattenberg, W. Kleinekofort, A. Pfenninger, B. Brutschy: Laser desorption mass spectrometry on thin liquid jets; Fresenius Journal of Analytical Chemistry 360 (1998), 745-749 

5) F. Sobott, W. Kleinekofort, B. Brutschy: Cation selectivity of natural and synthetic ionophores probed with laser induced liquid beam mass spectromenty; Analytical Chemistry 69 (1997); 3587-3594 

4) W. Kleinekofort, M. Schweitzer, J. W. Engels, B. Brutschy: : Analysis of double-stranded oligonucleotides by laser induced liquid beam mass spectrometry ; International Journal of Mass Spectrometry and Ion Processes 163 (1997); 1L-4L 

3) W. Kleinekofort, A. Pfenninger, T. Plomer, C. Griesinger, B. Brutschy: Observation of noncovalent polypeptide dimers using laser induced liquid beam ionization/desorption; International Journal of Mass Spectrometry and Ion Processes 156 (1996); 195-202 

2) W. Kleinekofort, J. Avdiev, B. Brutschy: A new method of laser desorption mass spectrometry for the study of biological macromolecules ; International Journal of Mass Spectrometry and Ion Processes 152 (1996); 135-142 

1) W. Kleinekofort, L. Germeroth, J. van den Broeck, D. Schubert, H. Michel: The light- harvesting complex B800/850 from Rhodospirillum Molischianum is an octamer ; Biophysica et Biochemica Acta 1140 (1992); 102-104

 

Forschungsinteressen

Extrakorporale Organersatzverfahren und automatisierte Systeme zur kontinuierlichen Organperfusion

Sensorbasierte Feedback-Algorithmen in der Medizintechnik

Charakterisierung von schwach gebundenen biomolekularen Komplexen und Biomarkern